Phospho sting molecular weight

WebHTRF Phospho and total STING enable accurate quantification of agonist-induced STING regulation. 400,000 THP1-R232 cells (from Invivogen) were plated under 25µl in 96-well … WebInvitrogen Anti-Phospho-STING (Ser366) Polyclonal, Catalog # PA5-105674. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and …

PMA (Phorbol myristate acetate) NF-kB Activator InvivoGen

WebFeb 13, 2024 · Keep in mind that each phosphorylation adds 80 Da to the molecular weight of the protein ( Walsh 2006 ). 3 Probing for Total Protein Using an antibody that detects the total level of your target protein enables you to determine the phosphorylated fraction relative to the total fraction. WebInterestingly, we found that STING interacts with both TBK1 and IRF3, and that mutations in STING that selectively disrupt its binding to IRF3 abrogate IRF3 phosphorylation without impairing TBK1 activation. These results suggest that STING functions as a scaffold to specify and promote IRF3 phosphorylation by TBK1. pool table slate seam filler https://ofnfoods.com

Western Blot Detection of Phosphorylation Events - Bio-Rad

WebSynthetic peptide corresponding to Human STAT3 (phospho S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P … WebMolecular Weight: 358.09 Match Criteria: Product Name, Keyword All Photos ( 1) Trolamine Synonym (s): Triethanolamine, 2,2′,2′′-Nitrilotriethanol, Tris (2-hydroxyethyl)amine Linear Formula: (HOCH2CH2)3N CAS No.: 102-71-6 Molecular Weight: 149.19 Beilstein No.: 1699263 Match Criteria: Keyword TMEM173 transmembrane protein 173 Synonyms: WebFeb 26, 2024 · p-Ser Antibody (16B4) is an IgM κ mouse monoclonal p-Ser antibody (also designated phosphorylated serine antibody, phospho serine antibody, or p-serine antibody) that detects the p-Ser protein of mouse, rat, human and canine origin by WB and IP. p-Ser Antibody (16B4) is available as the non-conjugated anti-p-Ser antibody. pool table slate leveling shims

Western Blot Detection of Phosphorylation Events - Bio-Rad

Category:STING Specifies IRF3 phosphorylation by TBK1 in the Cytosolic …

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Phospho sting molecular weight

Phosphate Blood Test: Purpose, High vs. Low vs. Normal Levels

WebFormula: C 36 H 56 O 8 Molecular weight: 616.8 g/mol Solubility: DMSO (5 mg/ml) Back to the top Contents 5 mg Phorbol myristate acetate (PMA) 1.5 ml endotoxin-free water Product is provided as a translucent film and shipped at room temperature. Back to the top Citations 2024 Sci Rep. DOI: 10.1038/s41598-022-27129-3 WebMolecular Weight 33, 35 Cell Signaling Technology 3 Trask Lane Danvers, Massachusetts 01923 United States Phone: 877-616-CELL Phone: 978-867-2388 Fax: 866-432-6112 Fax: 978-867-2488 Company Profile Website: http://www.cellsignal.com/ Website: Technical Support Citations (201)

Phospho sting molecular weight

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WebKeep in mind that each phosphorylation adds 80Da to the protein molecular weight (Walsh 2006). 3 Probing for the total protein Using an antibody that detects the total level of your … WebPhospholipids [1] are a class of lipids whose molecule has a hydrophilic "head" containing a phosphate group and two hydrophobic "tails" derived from fatty acids, joined by an alcohol residue (usually a glycerol …

WebHTRF Phospho and total STING enable accurate quantification of agonist-induced STING regulation. 400,000 THP1-R232 cells (from Invivogen) were plated under 25µl in 96-well plates in complete culture medium. The cells were stimulated with increasing concentrations of 2’3’cGAMP (5µL for 4 hours). WebProduct Details Product Specific Information This antibody detects endogenous protein at a molecular weight of 47 and 55 kDa. Purity is >95% by SDS-PAGE. Target Information IFR3 (interferon regulatory factor 3) is a …

WebJul 7, 2024 · The Stimulator of Interferon Genes (STING) pathway initiates potent immune responses upon recognition of DNA. To initiate signaling, serine 365 (S365) in the C-terminal tail (CTT) of STING is phosphorylated, leading to induction of type I interferons (IFNs). WebPhospho-STING (Ser366) (D7C3S) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser366. Species Reactivity: Human Source / …

WebPhosphophyllite (from Ancient Greek phyllon 'leaf', and phosphate) is a rare mineral with the chemical formula Zn 2 Fe(PO 4) 2 ·4H 2 O, composed of hydrated zinc phosphate. It is …

WebMolecular Weight phospho-AKT - 60 kDa (Figure 1) Figure 1. WB: HEK-293 cell lysate was subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) and 66444-1-Ig (AKT-phospho-S473 antibody) at a dilution … shared ownership or help to buyWebMay 28, 2024 · We observed the reduced expression of phosphorylated STING at 4 and 6 hours after diABZI treatment along with reductions in the higher–molecular weight … shared ownership pottonWebJun 13, 2024 · a WT and Sting gt/gt MEFs were infected with or without HSV-1 (MOI = 0.5) for 8 h. Sting was immunoprecipitated (IP) from the MEF lysates using a rabbit anti-Sting antibody. The immunoblots indicate Sting co-immunoprecipitation with Ubxn3b and Trim56 after viral infection. WCE whole-cell extract. pool tables makro south africaWebApplications. 1/500. 1/2000 - 1/20000. Predicted molecular weight: 88 kDa.Can be blocked with STAT3 (phospho Y705) peptide (ab179551). Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls. 1/20. 1/50 - 1/100. shared ownership over 55 schemeWebPhosphate is what you get when you combine the mineral phosphorus with oxygen. When you eat certain foods -- such as beans, nuts, cereal, milk, eggs, beef, chicken, and fish -- … pool tables kitchener waterlooWebWestern Blot analysis of Phospho-cGAS (Ser420) in various cell lines using a Phospho-cGAS (Ser420) Polyclonal antibody ( Product # PA5-121350) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of … pool table slate backingWebDec 24, 2024 · To develop a new chemical entity for STING activator to improve cyclic GMP-AMP (cGAMP)-induced innate immune response, we identified KAS-08 via the structural modification of DW2282, which was previously reported as an anti-cancer agent with an unknown mechanism. shared ownership panorama